| Type of microorganism |
Yeast |
| Microorganism name |
Komagatella phaffi (Pichia pastoris)
|
| Average yield in organism |
1.5-2 g/L (average values from studies cited before) |
| General temperature range |
28-30 °C (average values from studies cited before) |
| General pH range |
pH 5-6.5 (average values from studies cited before) |
| Growth rate (µ) |
|
| Ease of genetic modification |
Easy genetic manipulation, a lot of tools present (Lv & Cai, 2025)
|
| Feedstock case studies (suitable substrates) |
Corn waste, sugarcane leaves, soybean hydrolysate, rice bran, wheat bran, cottonseed hull, sugar beet molasses, bioethanol liquid waste, kitchen waste (Ergün et al., 2022)
|
| Downstream purification processing complexity (isloation, lysis, purification) * |
Possibility of adding a secretion signal to the protein, so easier purification & less downstream processing needed. (Spohner et al., 2015)
|
| Advantages |
Availability of strong, inducible promotors (like AOX1), easy genetic manipulation, low levels of host protein secretion,availibility of glycoeingineerd strains (Spohner et al., 2015)
|
| Challenges (Key limitations, risk factors) |
The strongest and most-used promotor is methanol-inducible, but methanol is toxic, flammable and explosive, making it hard to use in industrial context. (Pan et al., 2022)
|
| Extra/remark |
|